Fig. 1

P2RX6 is highly expressed and associated with RCC poor prognosis. a 628 DEGs’ Go pathway enrichment analysis. b qRT-PCR assay validation of 29 DEGs mRNA expression in 10 paired clinical tumor compared with para-tumor samples. c TCGA database analysis revealed P2RX6 expression is higher in stage III and stage IV (n = 207) compared with stage I and stage II (n = 325) (**P = 0.0035). d TCGA database analysis revealed P2RX6 expression is higher in T3 and T4 (n = 191) compared with T1 and T2 (n = 343) (**P = 0.0019). e TCGA database analysis revealed P2RX6 expression is higher in M1 (n = 79) compared with M0 (n = 424) (**P = 0.0077). (F-L) Human clinical studies for P2RX6 expression in RCC patients. f Human clinical samples analysis revealed P2RX6 expression is higher in higher RCC pathological stage samples. g Violin plot quantitative analysis for Fig. 1f. h Kaplan-Meier analysis for different P2RX6 protein expression in RCC patients (**P = 0.0018). i Representative IHC image of P2RX6 expression in Non and Meta group tissue samples. j Violin plot quantitative analysis for Fig. 1i. k Univariate Cox regression analysis and forest map. l Multivariate cox regression analyses and forest map. Non means non-metastatic group, and Meta means metastatic group. * P < 0.05, ** P < 0.01