Fig. 2

P2RX6 mediated the ATP-enhanced RCC migration/invasion. a WB validation of P2RX6 protein expression was elevated in multiple RCC (SN12-PM6, OS-RC-2, 786-O, SW839, A498) vs. normal renal proximal tubule epithelial cell line HK-2. b qRT-PCR assay validation of P2RX6 mRNA level knocking-down efficiency and c WB validation of P2RX6 protein level knocking-down efficiency when knocking down using sh-P2RX6^#1, sh-P2RX6^#2 in SN12-PM6 cells. d Migration assay after using 2 specific P2RX6 shRNAs in SN12-PM6 cells treated with ATP, PLKO.1-vector as control. e Quantitative analysis for Fig. 2D. f Transwell assay were performed after using 2 specific P2RX6 shRNAs in SN12-PM6 cells treated with ATP, PLKO.1-vector as control. g Quantitative analysis for Fig. 2f. h WB validated P2RX6 was overexpressed when using P2RX6-cDNA in 786-O and OS-RC-2 cells. i Migration assay after P2RX6 overexpression in 786-O and OS-RC-2 cells treated with ATP, pWPI-vector as control. j Quantitative analysis for Fig. 2i. k Transwell assay after P2RX6 overexpression in 786-O and OS-RC-2cells treated with ATP, PWPI-vector as control. l Quantitative analysis for Fig. 2k. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001