Fig. 4
Inhibition of ERK1/2 facilitated PSCs atrophy and induces p16, α-SMA. a Microphotograph of PSCs after treatment with DMSO and/or ERK inhibitor. Scale bars = 100 μm. b Viability of PSC1 and (c) PSC2 cells, as determined by CellTiter-Glo luminescent cell viability assay after 72 h’ treatment with indicated concentrations of ERK inhibitor; IC50 values are indicated. d qRT-PCR of PSCs shows mRNA expression changes after ERK inhibitor treatment. *P < 0.05, **P < 0.01, ***P < 0.001. e The indicated protein levels of PSCs were evaluated after treatment with DMSO as control and/or ERK inhibitor SCH772984 at IC50 value