Fig. 3

Combination of curcumin and gefitinib markedly depresses the receptor tyrosine kinase signaling pathways in gefiinib-resistant NSCLC cells. a H157 cells were treated with 5 μM gefitinib, or 5 μM curcumin alone, or the two combination for 48 h. Cell lysates and total mRNA were prepared and subjected to immunoblotting and RT-PCR analysis for detecting proteins and mRNA as indicated. b The combination of curcumin (5 μM) and gefitinib (5 μM) time-dependently suppressed expressions of proteins and mRNAs as indicated. c H157 and H1299 cells were treated with gefitinib (5 μM), or curcumin (5 μM) alone, or the two combination for 48 h. The whole-cell lysates were prepared and subjected to immuoblotting analysis for detecting phosphorylations of ERK1/2, MEK1/2, AKT and S6K proteins. Similar results were obtained from three independent experiments. Typical immunoblots were presented in the Figure