Fig. 7

Over-expressed ABI3BP and silenced MALAT1 and EZH2 impede GBC cell proliferation, migration, invasion and tumorigenic potential while accelerate senescence. a, representative images of resected tumors from nude mice and tumor weights. b, the relative expression of ABI3BP determined by immunohistochemistry (400 ×). c, the relative expression of H3K27me3 relative to β-actin determined by Western blot analysis. d, the relative expression of Ki67 and PCNA relative to β-actin determined by Western blot analysis. e, the cell migration detected by scratch test. f, the cell invasion detected by Transwell assay (200 ×). g, the cell senescence detected by SA-β-gal staining (400 ×). * p < 0.05 vs. nude mice treated with vector-NC or sh-NC. The data was measurement data and expressed as mean value ± standard deviation. Comparison was analyzed by one-way analysis of variance among multiple groups, followed by Turkey’s post-hoc test. The experiment was repeated 3 times. ABI3BP, ABI family member 3 binding protein; MALAT1, metastasis associated lung adenocarcinoma transcript 1; EZH2, enhancer of zeste homolog 2; GBC, gallbladder cancer; H3K27me3, trimethylation of lysine 27 on histone H3 protein subunit; PCNA, proliferating cell nuclear antigen; SA-β-gal, senescence-associated β-galactosidase; NC, negative control