Fig. 2

miR-126-3p replacement inhibits proliferation of dabrafenib-resistant cells. a, b A375R cells were transiently transfected with 50 nM of the indicated pre-miRs (a) or anti-miRs (b) or the appropriate controls, cultured for six days and then assayed for proliferation by the MTT assay. Data are expressed in terms of percentage of cell growth as compared to pre-miR-CTRL- or anti-miR-CTRL-transfected cells. Each value represents the arithmetic mean of three independent experiments performed with triplicate samples. Bars, SEM. ^*P<0.05 A375R/pre-miR-126-3p vs A375R/pre-miR-CTRL. c qRT-PCR analysis of miR-126-3p expression in SK-Mel28 and SK-Mel28R cells. The data were normalized to the level of RNU44 in each sample and expressed as 2^-ΔCtx10⁵ values. Each value represents the arithmetic mean of four independent experiments performed with duplicate samples. Bars, SEM. ^*P<0.05, SK-Mel28R vs SK-Mel28. d SK-Mel28R cells were transiently transfected with 50 nM pre-miR-126-3p or pre-miR-CTRL, cultured for six days and then assayed for proliferation by the MTT assay. Data are expressed in terms of absorbance at 595 nM. Each value represents the arithmetic mean of four independent experiments performed with triplicate samples. Bars, SEM. ^**P<0.01, SK-Mel28R/pre-miR-126-3p vs SK-Mel28R/pre-miR-CTRL.