Fig 6

Enforced expression of miR-126-3p delays the development of resistance to dabrafenib. a SK-Mel28 cells were seeded into 96-well plates and every eight days transfected with 50 nM pre-miR-126-3p (pre-126-3p) or pre-miR-CTRL (pre-CTRL) and treated with 100 nM dabrafenib (DAB) or DMSO. Cell cultures were photographed and processed for quantitative analysis of proliferation on day 0 (i.e after the first transfection), 8, 56 and 96. Images from a representative experiment are shown. b Quantitative analysis of proliferation of cell cultures described in (a). Crystal violet was solubilized and absorbance was read at 595 nm. Each value represents the arithmetic mean of two independent experiments performed with triplicate cultures. Bars, SEM. ^**P<0.01, pre-miR-126-3p vs matched pre-miR-CTRL; ^§§P<0.01, pre-miR-CTRL/DAB/Day 8 vs pre-miR-CTRL/DMSO/Day 8; ^††P<0.01, pre-miR-126-3p/DAB/Day 8 vs pre-miR-126-3p/DMSO/Day 8; ^##P<0.01, pre-miR-CTRL/DAB/Day 56 vs pre-miR-CTRL/DAB/Day 8; ^¶¶P<0.01, pre-miR-CTRL/DAB/Day 96 vs pre-miR-CTRL/DAB/Day 56; ^‡pre-miR-126-3p/DAB/Day 56 vs pre-miR-126-3p/DAB/Day8.