Fig. 2

Reducing lncRNA DLX6-AS1 inhibits self-renewal, amplification, and proliferation of LCSCs. a, expression of LCSCs markers at mRNA levels detected by RT-qPCR; b and c, expression of LCSCs markers at protein levels detected by western blot analysis; d, spheroid formation ability of LCSCs detected by Onco-spheroid formation assay, scale bar = 25 μm; e, colony formation ability of LCSCs detected by soft agar colony formation (SACF) assay, scar bar = 50 μm; f, proliferation of LCSCs detected by EdU staining, scale bar = 50 μm;^*, p < 0.05, vs. the blank group; the statistical data were expressed as mean value of standard error and analyzed by one-way ANOVA; ANOVA, analysis of variance; the experiment was conducted 3 times; RT-qPCR, reverse transcription quantitative polymerase chain reaction; LCSCs, liver cancer stem cells; LncRNA, long non-coding RNA; DLX6-AS1, DLX6 antisense RNA 1; LCSCs, liver cancer stem cells