Fig. 2

Effects of MnSOD shRNA or cDNA transduction on carcinogenicity and stemness in HCSLCs or MHCC97H cells. HCSLCs were transduced with Ad-GFP or Ad-shMnSOD. a Immunoblotting performed with anti-MnSOD and anti-FoxM1 antibodies, with β-actin antibodies used as a loading control; b and c Formed spheres and colonies (Scale bar, 200 μm); d Immunoblotting for CD133, CD44 and ALDH1, Bmi1, Nanog and Oct4; e The relative luciferase activity of FOXM1 promoter fragment in HCSLCs untreated (Mock), or transduced with Ad-GFP and Ad-sh MnSOD, respectively. MHCC97H cells were transduced with Ad-GFP or Ad-MnSOD. f Immunoblotting performed with anti-MnSOD and anti-FoxM1 antibodies; β-actin antibodies were used as a loading control; g and h Formed spheres and colonies (Scale bar, 200 μm); i Immunoblotting for CD133, CD44, ALDH1, Bmi1, Nanog and Oct4; j The relative luciferase activity of FOXM1 promoter fragment in MHCC97H cells untreated (Mock), and transduced with Ad-GFP and Ad-MnSOD, respectively