Fig. 5

Effects of isovitexin combined with FoxM1 shRNA or cDNA on carcinogenicity and stemness in HCSLCs or MHCC97H cells. HCSLCs were transduced with Ad-GFP and Ad-shFoxM1, respectively, incubated with or without isovitexin (ISOV; 10 μM). a Immunoblotting performed with anti-MnSOD and anti-FoxM1 antibodies, with β-actin antibodies as a loading control; b and c Formed spheres and colonies (Scale bar, 200 μm); d Immunoblotting for CD133, CD44, ALDH1 (D), Bmi1, Nanog and Oct4 amounts in HCSLCs transduced with Ad-GFP and Ad-shFoxM1, respectively, in the absence or presence of isovitexin. MHCC97H cells were transduced with Ad-GFP and Ad-FoxM1, respectively, and incubated with or without isovitexin. e Immunoblotting performed with anti-MnSOD and anti-FoxM1 antibodies, with β-actin antibodies as a loading control; f and g Formed spheres and colonies (Scale bar, 200 μm); h Immunoblotting for CD133, CD44, ALDH1, Bmi1, Nanog and Oct4 in MHCC97H cells transduced with Ad-GFP and Ad-FoxM1, respectively, cultured in the absence or presence of isovitexin