Fig. 4

Interplay between SPOCK1 and epithelial-to-mesenchymal transition (EMT) marker expressions is involved in apigenin (API)-mediated inhibition of cell motility. a Western blot analysis of the protein level of SPOCK1 in PNT2 prostate epithelial cells and LNCaP, DU145, PC-3, and PC-3 M prostate cancer cell lines. The protein level of SPOCK1 was higher in PC-3 M cells. b A Matrigel invasion assay was performed to study the invasive abilities of PC-3 and PC-3 M cells. Quantitative results of counting invaded cells in a 200× field. c SPOCK1 expression was assessed by a Western blot analysis in PC-3 and PC-3 M cells after treatment with various concentrations of API for 24 h. d-f Influences of SPOCK1 on EMT-related marker expressions (d) and invasive abilities of PC-3 M (e) and PC-3 cells (f) in response to API