Fig. 1

Colorectal cancer EVs increased BRCA1-KO fibroblasts proliferation and reduced their cell mortality. BRCA1-KO fibroblasts were cultured for 3 weeks in the presence of EVs isolated from control human sera, or cancer patient sera (a, c), and control medium or EVs isolated from HT-29 cell line conditioned medium (b, c). Cells were analyzed for their growth potential (a, b) and cell viability (c). a and b Population doublings capability was calculated at every passage. Data in inserts and column graphs represent cumulative population doublings at the end of the treatment periods. Column graphs represent pooled data from 10 control vs. 13 cancer patient sera (a), and from 5 control vs. 5 independent batches of HT29 conditioned media (b). Data are mean ± SD. P values are represented on the column graphs. (c) Treated cells were analyzed for cell viability using AnnexinV and 7-AAD staining followed by flow cytometry analyses. Cells were gated (G1) in a biparametric FSC/SSC graph. The percentage of apoptotic (AnnexinV positive and 7AAD negative) cells were plotted for comparison. Column graphs represent pooled data from 5 control vs. 5 cancer patient sera, and from 4 control vs. 4 independent batches of HT29 conditioned media. Data are mean ± SD. P values are represented on the column graphs