Fig. 3

PLEK2 interacted with EGFR. a Total cell lysates extract from FLAG-PLEK2 stably expressed cells were subjected to affinity purification and mass spectrometry analysis of PLEK2-associated proteins were performed. b Interaction between exogenous PLEK2 and EGFR by Co-IP analyses in 293 T cells. c Interaction between endogenous PLEK2 and EGFR by Co-IP analyses in NOZ cells. d, e Mapping of the binding site of PLEK2/EGFR by Co-IP analyses. f Immunofluorescence assays in GBC-SD cells. The localization of PLEK2 was detected by confocal laser scanning microscopy as indicated. g Co-IP of overexpressed Flag-PLEK2 and EGFR in 293 T cells treated with 50 ng/mL EGF at indicated times