Fig. 1

Alterations in CHTM1 levels affect sensitivity of lung cancer cells to glucose/glutamine starvation. (a) Western blot analyses showing CHTM1 knockdown in A549 and H460 lung cancer cells using three independent constructs. (b&c) CHTM1 knockdown and scrambled A549 and H460 lung cancer cells were glucose/glutamine starved for 6 h and 48 h respectively. Relative cell proliferation by MTT assay (Left panels), crystal violet staining (Middle panels) and representative phase-contrast photomicrographs (Right panels) showing decreased cell survival in CHTM1 knockdown cells compared to scramble cells under glucose/glutamine deprived condition. (d) CHTM1 knockdown and scramble A549 lung cancer cells were treated with 50 mM metformin for 48 h. Left, middle and right panels depict relative cell proliferation (MTT assay), crystal violet staining and representative phase-contrast photomicrographs respectively. CHTM1 knockdown cells show decreased cell survival following metformin treatment in comparison to metformin-treated scramble cells