Fig. 4

PTENP1 is a direct target of miR-26a and a positive regulator of PTEN. a Relative miR-20a expression was identified by qRT-PCR between BC tumor tissues and corresponding nontumor tissues. b Relative miR-20a expression was detected in MCF-10A and BC cell lines. c The negative correlation between PTENP1 and miR-20a was analyzed. d The predicted sequence aligment was shown, and dual-luciferase reporter assay confirmed the direct binding between PTENP1 and miR-20a. e Overexpressed miR-20a enhanced PTENP1 level in transfected MCF-7 and T47D cells. f MiR-20a inhibitor promoted PTENP1 expression in transfected MDA-MB-231, MCF-7/ADR and T47D/ADR cells. g The co-precipitated RNA was identified by RNA immunoprecipitation experiment. PTENP1 and miR-20a were shown as fold enrichment in Ago2. h The negative correlation was confirmed by Spearman’s correlation analysis. i The predicted binding sites were presented. The directed binding was confirmed by dual-luciferase reporter assay. j Downregulation of PTEN was detected in miR-20a mimic transfected MCF-7 and T47D cells. k Inhibition of miR-20a increased PTEN expression in MDA-MB-231, MCF-7/ADR and T47D/ADR cells. Data are the means ± SD of triplicate determinants (*P < 0.05)