Fig. 1

USP13 is a common target of miR-130b/301b, and expression of USP13 is significantly elevated in BC tissues. Expression of USP13 (a), miR-130b-3p (b) and miR-301b-3p (c) was detected in 50 pairs of normal bladder mucosa tissues and bladder cancer tissues by real-time PCR analysis. d and e. Pearson’s correlation coefficient test was performed to evaluate the correlation between expression of USP13 and miR-130b-3p/miR-301b-3p in 50 bladder cancer tissue specimens. The Transcriptional (f and g) and translational (h) levels of USP13 were measured in miR-130b/301b overexpressed or knocked down BC cells by real-time PCR analysis and western blotting analysis, respectively. i. The common seed sequence of miR-130b-3p and miR-301b-3p, and the miRNA response element on the 3’UTR region of USP13 gene. The mutations for USP13 3’UTR and agomir of miR-130b/301b were listed. j. The luciferase activity was measured in miR-130b/301b overexpressed 5637 cells co-transfected with luciferase construct harboring wild type 3’UTR or mutant type 3’UTR of USP13 gene. k. Luciferase activity was measured in 5637 cells co-transfected with luciferase construct harboring wild type 3’UTR of USP13 and miR-130b/301b agomir or mutant miR-130b/301b agomir. For real-time PCR, β-actin and U6 snRNA were used as the internal control for mRNA and miRNA, the Ct values for each group were compared using the the 2-ΔΔCt method. *P < 0.05 and **P < 0.01, as determined by Student’s T-test