Fig. 2
From: LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer
Silencing of CASC9 by GapmeR inhibits CRC cell growth and promotes cell apoptosis in vitro. HCT-116 and SW620 cells were transfected with Antisense LNA GapmeR targeting CASC9 (GapC9) or control GapmeR (GapNC). a, b Cell proliferation was determined by colony-formation assay (a) and, at the indicated time points, by MTS assay (b). c Cell cycle was analyzed by flow cytometry. The percentages of cells in G0/G1, S, and G2/M phases are shown. d Cell apoptosis was increased in CASC9-knockdown cells as determined by flow cytometry of cells with Annexin V-FITC/PI double staining. The data are presented as the mean ± s.d. **P < 0.01, ***P < 0.001, ****P < 0.0001 by Student’s t-test (a, c, d) or two-way ANOVA (b)