Fig. 1
PEITC inhibits cell proliferation, induces apoptosis and reactivates p53 mutants in prostate cancer cell lines. LAPC-4 (p53R175H) and VCaP (p53R248W) cells were treated with DMSO or PEITC for 24 h. a Percent cell proliferation was determined by the WST-1 assay, and b Apoptosis was measured by Annexin-V staining by flow cytometry using a BD LSRFORTESSA instrument. (**p ≤ 0.009 and *p ≤ 0.01). c Immunoprecipitation of the p53 mutant protein from PEITC treated LAPC-4 and VCaP cell lysates by using p53 mutant-specific antibody PAB240 and detected by a general anti-p53 (FL393) antibody. Input lysates were probed with a general anti-p53 (DO-1) antibody. Blots were stripped and reprobed with anti-GAPDH antibody. d qRT-PCR of p53 regulated genes in LAPC-4 and VCaP cells treated with DMSO or 8 μM PEITC for 4 h. (***p ≤ 0.0001 and **p ≤ 0.007). e LAPC-4 and VCaP cells were treated with DMSO or the indicated concentration of PEITC for 4 h. The cell lysates were resolved by SDS-PAGE, probed with p53 DO-1 antibody and reprobed with anti-GAPDH antibody