Fig. 4

PEITC acts in a p53 mutant type-dependent manner. a Western blot of the isogenic PC-3 cells harboring indicated plasmids. The cell lysates were resolved by SDS-PAGE, probed with p53 DO-1 antibody and reprobed with anti-GAPDH antibody. b Effects of PEITC on proliferation of PC-3 cells transfected with indicated plasmids after 24 h. Percent cell proliferation was determined by the WST-1 assay. c qRT-PCR of p53 regulated genes in PC-3 transfected cells treated with DMSO or 8 μM PEITC for 4 h. (***p ≤ 0.0000, **p ≤ 0.001 and *p ≤ 0.02)