Fig. 6

Modulation of protein levels by irradiation (IR) in C-4I cells transiently transfected with siC and siSTAT1. a 24 and 48 h post 2Gy IR C-4I cells were harvested and whole cell lysates (30 μg) were loaded into SDS-PAGE, followed by Western Blot with specific antibodies. Actin was used as loading control. NT: Non-treated; IR: 2 Gy γ-rays. Data are representative of at least three experiments. b Representative pictures showing merged fluorescent images of ANXA2 or PARP1 expression (red) and DAPI staining of DNA (blue) in siC and siSTAT1 C-4I cells, 24 h post-IR (magnification 100x)