Fig. 2

Overexpression of USP3 promotes the invasive and metastatic abilities of GC cells. a Comparison of the invasion potential of GC cells transfected with vector and USP3. b & (c) Representative images of the wound-healing assay in MGC-803 and BGC-823 cells. d & (e) The effect of RNA interference (RNAi) on USP3 gene mRNA expression and the invasive and migration potential of human GC cell lines. f Morphology of pooled cells stably transfected with vector or USP3 as visualized by phase-contrast microscopy. g E-cadherin and Vimentin expression was detected by cell immunofluorescence in BGC-823 cells. h Expression of epithelial markers and mesenchymal markers in vector- or USP3-transfected cells was assessed by Western blot. GAPDH was used as a loading control. Scale bars represent 50 μm in (f) and 20 μm in (g)