Fig. 2

LSH induces GINS4 expression and binds with GINS4 transcript. a-b Western blotting analysis of LSH and GINS4 in LSH-overexpressing (a) PC9 and H358, and HBE (b) cells (n = 3). c Western blotting analysis of LSH and GINS4 in LSH-depleted A549 cells (n = 3). d qRT-PCR analysis of LSH expression in 79 lung cancer tissue samples and corresponding paired normal lung tissue samples. e Correlation between LSH and GINS4 expression was analyzed. (F-G) qRT-PCR analysis was performed to determine the relative binding of LSH to the GINS4 mRNA in LSH-overexpressing (f) PC9 and (g) H358 cells and in (h-i) 293 cells overexpressing truncated LSH fragments (h). i Results were normalized using those obtained for respective IgG controls, with the 18S rRNA gene as a loading control (n = 3)