Fig. 3

LSH increases GINS4 stabilization through 3’UTR of GINS4. a-c LSH-overexpressing (a) PC9 and (b) H358 cells and (c) LSH-knockout A549 cells were treated with actinomycin D (5 μg/mL) for the indicated time. GINS4 and β-actin mRNA stability was assayed by performing qRT-PCR, with the 18S rRNA gene as a loading control (n = 3). d Luciferase activity in 293 cells coexpressing a luciferase reporter vector encoding the GINS4 3′UTR and LSH (n = 4). e Luciferase activity in LSH-depleted A549 cells expressing a luciferase reporter vector encoding the GINS4 3′UTR (n = 4). *P < 0.05, **P < 0.01, and ***P < 0.001