Fig. 7

LAMP1 involves in UBL4A-mediated anti-tumor effects. a-f The role of LAMP1 in UBL4A-induced migration and invasion was demonstrated by transwell assay in four PDAC cell lines (original magnification, 10×) (bars, 25 μm). g The proliferative capacity of SW1990 was determined by EdU retention assays (original magnification, 20×) (bars, 50 μm) in four different groups (Vector, lv-UBL4A-Flag, siLAMP1 and lv-UBL4A-Flag+siLAMP1). h Representative fluorescent photographs of Edu retention assay in BxPC-3 were subjected to shCtrl, LV-shUBL4A, LAMP1 plasmid and LV-shUBL4A + LAMP1 plasmid groups. i The ratio of DNA replication was calculated. j-l Wound healing assay was performed to detected the role of LAMP1 in UBL4A-mediated metastasis in CFPAC-1 and BxPC-3 (original magnification, 10×) (bars, 25 μm). The statistical significance between different groups was calculated with Student t- test. Data are shown as the mean ± SD of three replicates; *P < 0.05, **P < 0.01; ***P < 0.001; ns: not significant