Fig. 3

MUC17 protects GC cells against the effects of inflammatory cytokines. a Increased CDX1 expression induced by IL1β and IL8 in AGS cells, as determined by western blotting. b Immunofluorescence showed the failure of IL1β and IL8 to increase MUC17 expression in AGS cells transfected with a shRNA targeting CDX1. Scale bars, 10 μM. c Significantly increased MUC17-si cell growth rates induced by IL1β and IL8 compared to Mock-si cells in an MTT assay. Error bars represent ± SD (*P < 0.05, ** P < 0.001). d Increased p65 translocation to the nucleus in MUC17-si cells induced by IL1β (upper) or IL8 (lower) compared to Mock-si cells, detected by immunofluorescence. Scale bars, 10 μM. e Upregulating pp65 expression in MUC17-si cells induced by IL1β (left) or IL8 (right) compared to Mock-si cells, detected by Western blotting. f The expression of MUC17 in early stage GC tissues (right) and adjacent normal tissues (left). Scale bar, 50 μM. g In MUC17 highly expressed GC tissues, IL8 expression was negatively correlated with p65 expressed in nuclear. Scale bar, 50 μM