Fig. 4

MUC17 inhibits GC cell proliferation through its EGF domain and following sequence. a Knock-down of MUC17 promoted GC cell proliferation in AGS cells measured by MTT assay. b Knock-down of MUC17 increased AGS cells proliferation measured by colony formation assay. c Knock-down of MUC17 promoted BrdU incorporation in AGS cells measured by BrdU incorporation assay. d Overexpression of truncated MUC17 inhibited MKN45 cells proliferation measured by MTT assay. e Overexpression of truncated MUC17 inhibited MKN45 cells proliferation measured by colony formation assay. f Overexpression of truncated MUC17 inhibited BrdU incorporation in MKN45 cells measured by BrdU incorporation assay. g Overexpression of T-MUC17 induced G2/M phase cell cycle arrest of MKN45 cells. h Knock-down of MUC17 decreased the expression of G2/M phase arrest marker (left) and overexpression of truncated MUC17 increased the expression of the markers (right). Error bars represent ± SD of three experiments (*, P < 0.05; **, P < 0.001)