Fig. 2
Knockdown of IPO5 inhibits the growth and migration of colorectal cancer cells. a Overexpression or knockdown efficiency of IPO5 was confirmed by western blotting. b IPO5 knockdown significantly inhibited cell growth on the basis of CCK8 and colony formation assay. Error bars represent the means ± SD of 5 or 3 independent experiments. ***p < 0.001. c Cell cycle was detected by flow cytometry after silence IPO5 expression. d The expression levels of Cycle-related proteins were detected by western blotting. e Knockdown of IPO5 increased apoptotic cells as analyzed by flow cytometry. f Expression of apoptosis protein markers were analyzed using western blotting. g Silencing of IPO5 reduced CRC cells migration was showed by transwell assays. Error bars represent the means ± SD of 5 different fields. *p < 0.05. h IPO5 knockdown inhibited growth of RKO xenografts in nude mice (n = 7). Right panel showed the tumor sizes change. The data were calculated as the mean tumor volumes ± SD for 7 samples. i Representative images of IHC staining and Ki67-positive Sections of subcutaneous tumors. Error bars represent the means ± SD of 5 different fields