Fig. 4

RASAL2 represent novel IPO5 cargo protein a CO-IP technology was used to identify IPO5 interaction proteins. After silver staining, specific protein bands were collected and then identified by mass spectrometry (MS). b Evaluation of the biological processes of the putative IPO5 binding partners. Candidates were conducted on the pathway analysis with Cytoscape softwar and Wikipathway database. c Subcellular fractionation was performed to illustrate that RASAL2 is one of the cargos of IPO5. Data represent the mean ± SD of 3 independent experiments. *P < 0.05. d CO-IP assays was used to further validation the interaction between RASAL2 and IPO5. e Representative fluorescence images of the location change of RASAL2 in the cells knockdown or overexpress IPO5