Fig. 5

IPO5 mediates RASAL2 nuclear transport by NLS a Putative NLS sequence segments of RASAL2 protein. b Immunofluorescence images exhibit the localizations of the exogenously expressed RASAL2 wild type and putative NLS mutant fused with HA in CRC cells. c The effect of WT and putative NLS mutant plasmid on the distribution of RASAL2 was tested by subcellular fractionation method in CRC cells. d The impact of NLS mutant on the interaction between RASAL2 and IPO5 was detected by CO-IP assays