Fig. 3

Characterization of the different cytotoxic effect of CDDP and CBDCA on TNBC cells. Dispersion graphs show the decreased viability of HCC70 (a) and HCC1937 (b) cells when treated with the indicated doses (μM) of CDDP for 24 h. Graphs in (c) and (d) report the diminished viability of HCC70 and HCC1937 cells, respectively, when treated with the indicated doses (μM) of CBDCA for 24 (blue line) and 48 (red line) hours. e Western blotting on whole cell extracts of HCC70 cells transfected with the Flag-NPM1 plasmid and relative control (EMPTY) compared to HCC1937 cells shows the amount of NPM1. On the right side of each panel, the Molecular Weights (MW), expressed in kDa, are indicated. On the left side of each panel, specific antibodies used in the immunoblotting are indicated. f Histogram reporting the viability of HCC70 cells overexpressing NPM1 upon CDDP treatment. Values express the mean viability ± SD from at least three independent replicates. Each value is normalized to the untreated condition. ***p < 0.001