Fig. 6

LEF1 activates TGF-β signaling and directly upregulates ID1 in HCC cells. a, b Western blot of GADPH (loading control), phosphorylated and total protein of Smad2 and Smad3, TGF-β1, ID1 in ECA109 and TE1 with LEF1 knockdown by shRNA. c, d Seven differentially expressed transcripts were validated by qRT-PCR in LEF11 overexpression ECA109 and TE1 cells. Data is shown as the mean ± SD, *P < 0.05, **P < 0.01. e The sequence logo of a potential LEF1 binding site in JASPAR and potential binding site in the ID1 sequence. f Construction of plasmid and overexpression of LEF1 significantly enhanced the relative luciferase activity compared with control group in ECA109 and TE1 cells, *P < 0.05, **P < 0.01. All experiments were performed in triplicate