Table 1 Sequence if PCR Primers Used in This Study
Gene | Forward primer sequence (5’ −> 3’) | Reverse primer sequence (5’ −> 3’) |
|---|---|---|
CD133 | GCCACCGCTCTAGATACTGC | TGTTGTGATGGGCTTGTCAT |
CD44 | GAGCATCGGATTTGAGA | CATACTGGGAGGTGTTGG |
ABCG2 | CAGGTTACGTGGTACAAGATGA | GATCAGTGATAAGCTCCATTCC |
KLF4 | CCATTACCAAGAGCTCATGC | GTGCCTGGTCAGTTCATCTG |
SOX2 | CAAGATGCACAACTCGGAGA | GCTTAGCCTCGTCGATGAAC |
NANOG | CTGCTGGACTGAGCTGGTTGCC | GCTGAGGCCTTCTGCGTCACA |
OCT4 | AGTGAGAGGCAACCTGGAGA | ACACTCGGACCACATCCTTC |
EPCAM | AATCGTCAATGCCAGTGTACTT | TCTCATCGCAGTCAGGATCATAA |
CXCR4 | ACTACACCGAGGAAATGGGCT | CCCACAATGCCAGTTAAGAAGA |
LEF1 | AGAACACCCCGATGACGGA | GGCATCATTATGTACCCGGAAT |
COMP | GATCACGTTCCTGAAAAACACG | GCTCTCCGTCTGGATGCAG |
BMP8A | AGAAAAGCAACGAGCTGCCG | GCCGCGGACGTCATCAAA |
ID1 | CTGCTCTACGACATGAACGG | GAAGGTCCCTGATGTAGTCGAT |
ID3 | GAGAGGCACTCAGCTTAGCC | TCCTTTTGTCGTTGGAGATGAC |
SMAD9 | CTAGGCTGGAAGCAAGGAGAT | GGGGAATCGTGACGCATTT |
CHRD | TTCGGCGGGAAGGTCTATG | ACTCTGGTTTGATGTTCTTGCAG |
DCN | ATGAAGGCCACTATCATCCTCC | GTCGCGGTCATCAGGAACTT |