Fig. 4

Half depletion of CDK5 reduces HCC tumor development in DEN-induced HCC mice. a Immunoblotting analysis of CDK5 protein in tumor(T) and non-cancerous surrounding tissues(N) of DEN induced HCC mouse model. t test,*P < 0.05. b Immunofluorescence of CDK5 in DEN induced HCC mouse model. c Specimens of liver obtained from DEN induced HCC model in wild type and half depletion of CDK5 mice after 10 months injection. Hematoxylin-eosin staining of tumor and corresponding para-tumor liver tissue was shown in the right panel. d Tumor numbers after 10 months injection of DEN was shown. t test, **P < 0.01. e Maximum diameters of tumor was shown, t test,*P < 0.05. f Immunostainings of tumor and para-tumor liver tissue staining for Ki67 in wild type and half depletion of CDK5 mice. Positive incidence of Ki67 was shown in the right panel. t test, ***P < 0.001