Fig. 8

Inhibition of miR-501-3p in macrophage exosomes represses tumor formation and metastasis in nude mice. a-d xenograft tumors and quantitative analysis of tumor mass after Mp-Exo + miR-501-3p antagomiR treatment (n = 7). M2 macrophages were transfected with NC antagomiR or miR-501-3p antagomiR, after which exosomes were extracted and injected into the nude mice. Tissue samples were collected after 4 weeks to detect relevant indicators. e-l HE staining analysis and quantitative analysis of liver and lung nodules after Mp-Exo + miR-501-3p antagomiR treatment (n = 7). m and n. RT-qPCR detection of the expression of miR-501-3p in subcutaneous tumor tissues and exosomes from the serum of nude mice in response to Mp-Exo + miR-501-3p antagomiR. o and p RT-qPCR detection of expression of tumor cell stemness-related genes and THFBR3 in mouse subcutaneous tumor tissues in response to Mp-Exo + miR-501-3p antagomiR. q-t Western blot analysis of migration, invasion and angiogenesis-related proteins as well as TGFBR3 protein in PANC-1 and BxPC-3 cells treated with Mp-Exo + miR-501-3p antagomiR. * p < 0.05 vs. Mp-Exo + NC antagomiR. The measurement data were expressed as mean ± standard deviation. Data between two groups were analyzed by independent sample t test. One-way ANOVA was employed for comparison among multiple groups. The data at different time points were compared using repeated measures ANOVA, followed by Tukey’s post-hoc test