Fig. 6

Caspase pathway was activated by the inhibition of p-STAT3 in Exo/R. a. RKO/P cells were exposed to 30 μM 5-FU and exosomes for 72 h, and then WB was used to detect the expression of p-STAT3, cleaved Caspase-9 and Caspase-3. b, c. High content analysis of active caspase-3 in RKO/P cells. Caspase-3 activity was calculated at 36 h (b) and 72 h (c) after cells exposed to 30 μM 5-FU, exosomes and 5 μM stattic. d. RKO/P cells were cultured with 30 μM 5-FU and exosomes in the presence of DMSO or 5 μM stattic for 56 h. WB was used to detect cleaved caspase-9, caspase-3 and PARP in RKO/P cells. * P < 0.05