Fig. 4

LUCAT1 contributes to stemness of BCSCs. a Expression of stemness markers (SOX2, Nanog and OCT4) was detected by qRT-PCR in stable sh-LUCAT1 transfected MCF-7 CSCs and T47D CSCs. b Expression of stemness markers (Nanog and SOX2) was detected in LUCAT1-silencing MCF-7 CSCs and T47D CSCs by WB. c Rate of the CD44 + CD24− cells in MCF-7 CSCs and T47D CSCs transfected with sh-LUCAT1 subpopulation by flow cytometry. Original magnification, × 400. Scale bars, 50 μm. d Self-renewal ability was detected in LUCAT1-silencing MCF-7 CSCs and T47D CSCs by sphere formation assays. Data are presented as the mean ± SD of three independent experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001