Fig. 4

Tac2-N activates NF-κB signaling pathway in lung cancer cells. a GSEA showed that high Tac2-N expression was positively correlated with NF-κB signaling in lung cancer. b H1975 cells were co-transfected with vector control or Tac2-N expression vector and NF-kB reporter plasmid. The activity of NF-κB was measured at 24 h after the transfection. c H1299 cells were co-transfected with negative control or Tac2-N shRNA and NF-kB reporter plasmid. The activity of NF-κB was measured at 24 h after the transfection. d Schematic illustration of full-length and truncated Tac2-N constructs. e H1975 cells were co-transfected with vector control or Tac2-N expression vector or truncation constructs and NF-kB reporter plasmid. The activity of NF-κB was measured at 24 h after the transfection. f qRT-PCR analysis of MMP7 and MMP9 expression in H1975 cells transiently transfected with the vector control or Tac2-N. ACTIN was used as an internal control. g The protein expression of MMP7 and MMP9 were detected by WB in H1975 cells. h qRT-PCR analysis of MMP7 and MMP9 expression in H1299 cells transiently transfected with the negative control or Tac2-N shRNA. ACTIN was used as an internal control. i The protein expression of MMP7 and MMP9 were detected by WB in H1299 cells. j p65 and p50 protein levels in nucleus and cytoplasm were analyzed by WB. k WB analysis showed that Tac2-N could upregulation phosphorylation level of IκB with downregulation expression of IκB. ACTIN was used as an internal control