Fig. 1

The effects of IDO1 on cell migration of GC. IDO1 expression was detected by Western blot (a) and qRT-PCR (b) in gastric epithelial cell line (GES-1) and GC cell lines (MGC-803, HGC-27, NCI-N87, AGS, Hs746T, SGC-7901 and MKN45). c IDO1 RNA level in 32 paired GC and normal tissues in TCGA database (P < 0.001). Western blot (d) and qRT-PCR (e) were used to detect IDO1 expression after transfecting IDO1 siRNAs for 2 days in AGS and SGC-7901 cells. MGC-803 and HGC-27 were transfected by IDO1-expressing eukaryotic plasmid for 2 days, and IDO1 was examined by Western blot (f) and qRT-PCR (g). h Effects of IDO1 expression on GC cell proliferation of AGS, SGC-7901, MGC-803 and HGC-27. IDO1 was knocked down by siRNA transfection, and upregulated by IDO1-expressing eukaryotic plasmid transfection. i Knockdown of IDO1 by siRNA transfection inhibited migration of AGS and SGC-7901 (200×). j IDO1 up-regulation by IDO1-expressing eukaryotic plasmid transfection promoted cell migration of MGC-803 and HGC-27 (200×). “*” represented comparing with “Normal”, “NC” or “Vector” group. *P < 0.05, **P < 0.01, ***P < 0.001