Fig. 3

Characterization of hsa_circ_0058124 in PTC cells. a Schematic diagram of the genomic location and splicing pattern of hsa_circ_0058124 (864 bp). b The existence of hsa_circ_0058124 was validated by RT-PCR in two PTC tumor tissue samples (T1 and T2) and two PTC cell lines (TPC-1 and K1). c Arrow represents the “head-to-tail” splicing sites of hsa_circ_0058124 according to Sanger sequencing. d qRT-PCR was used to determine the abundance of hsa_circ_0058124 and linear FN1 mRNA in TPC-1 cells treated with RNase R (normalized to negative control). e RNA fluorescence in situ hybridization (FISH) showed the localization of hsa_circ_0058124 in TPC-1 cells. DAPI solution was used to stain the nuclei. f qRT-PCR data indicating the abundance of hsa_circ_0058124 in either the cytoplasm or nucleus of TPC-1 and K1 cells. The data represent the mean ± S.E.M. from three independent experiments. ^*P < 0.05; ^**P < 0.01