Fig. 5

PLCε depletion decreases the phosphorylation of Twist1 on serine 68 and reduces stability of Twist1 protein via MAPKs. a, b Western blotting and protein quantification analyses detected the expression of PLCε, phosphorylation of serine 68 of Twist1 (pSer68) and Twist1 after knockdown PLCε in PCa cells. c, d Western blotting and protein quantification analyses detected the expression of B-Raf, p-MEK, t-MEK, p-ERK, t-ERK, MEKK3, MEK4, p-P38, P38, MEKK2, p-JNK and t-JNK in sh-PLCε-infected PC3 cells. e, f Western blotting and protein quantification analyses detected the expression of PLCε, Twist1, pSer68, B-Raf, p-MEK, t-MEK, p-ERK and t-ERK in PC3 cells treated with 1 nM trametinib. g, j Relative mRNA of PLCε and Twist1 in PC3 cells treated with trametinib or JNK-In-8. h, i Western blotting and protein quantification analyses detected the expression of PLCε, Twist1, pSer68, MEKK2, p-JNK and t-JNK in PC3 cells treated with 5 nM JNK-In-8. Data were represented as mean ± SD. of three individual experiments. *p < 0.05, **p < 0.01, and *** p < 0.001 vs. Controls