Fig. 6

HMGB1-mediated autophagy regulates the imaging of ^99mTcO4⁻ and NIS expression in tumor-bearing nude mice in vivo (a) Nude mice received a subcutaneous injection of HMGB1 shRNA and control shRNA cells and then food was withdrawn at 18 h before experimentation when murine tumor was > 150 mm³. Wholebody scintigraph of bearing-tumor mice was performed at 10 min after a tail injection of ^99mTc-pertechnetate. 1: thyroid gland, 2: transplanted tumor, 3: stomach. Arrows indicated that transplanted tumor was not visualized; (b) Biodistribution of ^99mTcO4 ^- in tumor-bearing nude mice (n = 3, *P < 0.01 vs. HMGB1 shRNA hunger group or control shRNA vehicle group); (c) Immunohistochemical staining of NIS was performed with isolated tumor at the end of experiment