Fig. 1

KIFC1 was identified as an oncogenic factor in HCC and is associated with poor survival and advanced stages. a The fold change of KIFC1 mRNA expression in 40 paired HCC and adjacent nontumor tissues and liver cancer dataset from TCGA database. Data are presented as the mean ± SD, * P < 0.01, ** P < 0.001. b KIFC1 protein expression in 12 pairs of HCC and adjacent nontumor tissues. c Expression of KIFC1 in HCC cell lines (HepG2, Hep3B, QGY-7701, BEL-7402, SMMC-7721, Huh-7, CRL-8024, and LM6) and immortalized hepatocytes MIHA and LO2 were examined by western blotting. d and e: IF and IHC assays demonstrated that KIFC1 was mainly located in the nucleus in both HCC cell lines and patient tissues.The bar indicated 10 μm. f Representative images of KIFC1 expression in TMA detected by IHC from both adjacent nontumor and HCC tissues. g Kaplan-Meier analysis indicated the correlation between high expression of KIFC1 and poor OS and DFS rates in HCC patients. h Survival curves from TCGA cohort validated that HCC patents with high levels of KIFC1 had shortened OS and DFS rates