Fig. 1

YBX1 is critical for RCC cell migration, invasion, and adhesion. The expression of YBX1 was detected after lentivirus transfection by real-time PCR (a) and western blot (b). β-actin was used as loading control, respectively. ACHN and A498 cells stably expressing shYBX1 were subjected to Migration (c) and Matrigel-coated invasion assays (d). Left panels: the representative images of migrated/invaded cells, Naïve: untreated; Scr: scramble control; shYBX1: YBX1 knockdown. Right panels: histograms, corresponding to left panels, show means ± SD of cell numbers from three independent assays. Statistically significant differences were indicated: ***, P < 0.001. NS: no significant difference