Fig. 1

Synthesis and characterization of PH1. a Synthesis of PEG₃₃₅₀-PEI₆₀₀-CyD. b H¹NMR analysis of PEG₃₃₅₀, PEI₆₀₀-CyD, and PEG₃₃₅₀-PEI₆₀₀-CyD. c Particle size of PH1/plasmid polyplexes at the N/P ratio of 20/1. d Zeta potential of PH1/plasmid polyplexes at the N/P ratio of 20/1. e In vitro transfection effciency assay of PH1 in 293 T cells. f Distribution analysis of YOYO-1 labeled pHGFK1 was performed with in vivo optical imaging system