Fig. 6

Depleting PTOV1 impaired stem cell-like properties via DKK1/β-catenin signaling. a GSEA analysis of the correlation between PTOV1 expression and the GO_ CANONICAL_WNT_SIGNALING_PATHWAY gene signature. b TOP/FOP Flash activity of the indicated cells. c Representative images of immunofluorescence staining of β-catenin in the indicated cells. d Real-time quantitative PCR detecting the expression of LEF1, AXIN2, MMP9and DKK1 in the indicated cells. e TOP/FOP Flash activity of the indicated cells transfected with NC or DKK siRNA. f Representative images of tumor sphere formation of the indicated cells transfected with NC or DKK siRNA. Error bars represent the mean ± SD from 3 independent experiments. *, P < 0.05; #, P < 0.01; $, P < 0.001, two-tailed, unpaired t-test