Fig. 3

HDAC1 and HDAC6 bind RUNX2 regulatory elements and control their transcriptional activation status. TPC1 and MDA-MB-231 were assessed for the binding of HDAC1 (a-b) and HDAC6 (c-d) to the regulatory elements of RUNX2 by ChIP assays. ChIP experiments were also performed to evaluate levels of H3K27Ac in RUNX2 regulatory regions 48 h after transfection with siRNA specific for HDAC1 (e-f) or HDAC6 (g-h). Histograms represent the average enrichment of the indicated genomic regions in the immunoprecipitated DNA expressed as percentage of the Input. All data are expressed as mean values +/− SEM of a technical triplicate and are representative of at least two independent experiments. * p < 0.05