Fig. 6

FLVCR1-AS1 regulated cell proliferation, migration and invasion through targeting YAP1 in OSC cells. a, b The plasmids of pcDNA-3.1/YAP1 were transfected into SKOV3 and OVCAR3 cell lines to achieve YAP1 overexpression. The transfection efficiency was verified using western blot assay. c Si-NC/si-FLVCR1-AS1 and pcDNA3.1/YAP1 were cotransfected into SKOV3 and OVCAR3 cell lines. CCK8 assay was used to determine the cell growth. d, e The cell migration and invasion in both SKOV3 and OVCAR3 cell lines were significantly reduced by FLVCR1-AS1 knockdown, while were partly restored in response to YAP1 overexpression (magnification 400×). The data were presented as mean ± SD of three independent experiments. **P < 0.01