Fig. 8

FLVCR1-AS1 promoted EMT process and in vivo tumor growth in OSC cells. a, b FLVCR1-AS1 knockdown significantly promoted the expression of E-cadherin but inhibited Snail and vimentin, whereas FLVCR1-AS1-overexpression led to the opposite results in OSC cells compared with control group. c, d The positive immunostaining SKOV3 cells of E-cadherin were increased, while vimentin was decreased when FLVCR1-AS1 was knockdown compared to the si-NC group; however, FLVCR1-AS1-overexpression led to the opposite results compared with controls in OSC cells. e Bioluminescence of Xenograft live imaging of mice at 4 weeks following intrabursal injection of si-FLVCR1-AS1 or si-NC transfected SKOV3 cells. f Tumor weight in mice primary ovaries injected with si-FLVCR1-AS1 or si-NC SKOV3 cells. Inhibition of FLVCR1-AS1 inhibits OSC cell tumor growth in vivo. g FLVCR1-AS1 knockdown significantly promoted the expression of E-cadherin but inhibited Snail and vimentin in mice tumors. h The mRNA expression of miR-513 in mice primary tumors injected with si-FLVCR1-AS1 or si-NC SKOV3 cells. i, j mRNA and protein expressions of YAP1 in mice primary tumors injected with si-FLVCR1-AS1 or si-NC SKOV3 cells. k The positive immunostaining cells of YAP1 and PCNA were decreased following FLVCR1-AS1 knockdown compared to the negative control group in mice primary tumors of ovary. **P < 0.01