Fig. 6

DTL promoted proliferation and motilities of lung and breast cancer cells. a-b Lung cancer cell line H1650 (a) and breast cancer cell line MDA-MB-468 (b) were used to establish DTL overexpression cell lines. Cell proliferation was examined by MTT. c-d Lung cancer cell line A549 (c) and breast cancer cell line MDA-MB-231 (d) were used to establish DTL silencing cell lines. Cell proliferation was examined by MTT. e-f Transwell and Matrigel assays were carried out in DTL overexpression cell lines H1650 (e) and MDA-MB-468 (f). g Transwell and Matrigel assays were carried out in DTL silencing cell line MDA-MB-231. h Nude mice were hypodermically injected with DTL overexpression cell line H1650. The nude mice were sacrificed by anesthesia with chloral hydrate and the transplanted cancer tissues were dissected out. i Tumor weights were measured after dissected. j The tumor volumes were measured during their growth process using ruler, calculating by the formula (length×width)²/2. k Nude mice were hypodermiclly injected with silencing cell line MDA-MB-231. The nude mice were sacrificed by anesthesia with chloral hydrate and the transplanted cancer tissues were dissected out. l Tumor weights were measured after dissected. m The tumor volumes were measured during their growth process and calculating by the formula (length×width)²/2. n-o Total numbers of nude mice with distant metastasis at 40 to 50 days after tail vein injection of DTL overexpression cell line BT549 and DTL silence cell line MDA-MB-231. Lung tissues were dissected out and made into paraffin sections after the nude mice were sacrificed. Distant metastasis were observed after hematoxylin and eosin (HE) staining. The lower panel showed the representative HE staining. The scale bars represent 50 μm. *, P < 0.05, **, P < 0.01 based on the Student t test. All results are from three or four independent experiments. Error bars, SD