Fig. 2

The effects of S1PR1 downregulation on cell proliferation and apoptosis of ESCC cells. a. CCK-8 assay analysis of kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. b. Flow cytometry analysis of cell cycle distribution proportion in kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. Representative images and average percentages of cells are shown. c. Colony formation assay of kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. d. Flow cytometry analysis of apoptotic cells in kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. The sum of Annexin V positive population and PI positive population is exhibited. e. Immunofluorescent staining of c-caspase3 of kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. f. Western blot analysis of S1PR1, PCNA, cyclinD1, Bcl-xL, c-caspase3 and GAPDH in kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively